Using the Southern blot technique, this quantified DNA can be isolated and examined further using PCR and RFLP analysis.
2.
The Southern blot technique is laborious, and requires large amounts of undegraded sample DNA . Also, Karl Brown's original technique looked at many minisatellite loci at the same time, increasing the observed variability, but making it hard to discern individual alleles ( and thereby precluding paternity testing ).
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